About the project
Infections of diabetic foot ulcers (DFUs) are a distressingly common complication of diabetes, frequently leading to lower-limb amputations. Recognising the urgent need for improved prevention and management strategies, our project employs a comprehensive approach combining whole genome sequencing and laboratory assays to elucidate the diversity of Escherichia coli (E. coli) strains associated with DFUs. By analysing the complete genetic information of these strains, we aim to unravel their phylogenetic relationships and identify potential virulence factors and antibiotic resistance mechanisms. Furthermore, through detailed phenotypic characterization, including biofilm formation assays and antibiotic susceptibility testing, we can assess the clinical relevance and pathogenic potential of these E. coli isolates. By integrating genomic and phenotypic data, our research aims to enhance our understanding of E. coli infections in DFUs, ultimately leading to the development of targeted prevention and management strategies. Ultimately, the outcomes of this study have the potential to significantly improve the health and well-being of individuals with diabetes by reducing the incidence of DFU-associated complications and mitigating the need for lower-limb amputations.
Funding
This work is funded by the University of Westminster/UKRI QR PhD Research Scholarship.
Contact
For further information contact the Principal Investigator Dr. Vincenzo Torraca at [email protected].